Fig. 6.

Constipation induced gut microbiota dysbiosis aggravated the cytokines disturbance in EAE mice. Serum samples of different treated mice were aseptically harvested on 25st day p.i. for circulating cytokines measurement. The level of TGF-β was determined using an ELISA Kit (a). The levels of GM-CSF (b), IL-10 (c), IL-17A (d), IL-17F (e), IL-21 (f), IL-22 (g) and IL-23 (h) were determined using a ProcartaPlex™ multiplex immunoassay. N = 6 in each group. Data are displayed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001