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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Constipation induced gut microbiota dysbiosis exacerbates experimental autoimmune encephalomyelitis in C57BL/6 mice

Fig. 4

Constipation induced gut microbiota dysbiosis aggravated Treg/Th17 and Treg17/Teff17 imbalance in peripheral lymphoid organs of EAE mice. Lymphocytes were harvested from the spleens and ILNs of all mice on 25st day p.i. and subsequently analyzed by flow cytometry. Mononuclear cells were first gated for lymphocytes followed by the gating of CD4+ T cells, and then analyzed for the percentage of Treg (CD4+Foxp3+) cells, Th17 (CD4+IL-17A+) cells, Treg17 (CD4+IL-17A+ Foxp3+) cells and Teff17 (CD4+IL-17A+ Foxp3−) cells. Representative flow data showed percentage of Treg, Th17, Treg17 and Teff17 cells in the spleens and ILNs gated on CD4 positive cells of three groups (a, d). Quantification of the percentage of Treg, Th17, Treg17, Teff17 cells in the spleens and ILNs from three groups (b, e). Quantification of ratio of Treg/Th17 and Treg17/Teff17 in the spleens and ILNs from three groups (c, f). N = 6 in each group. Data are displayed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001

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