Fig. 3

Constipation induced gut microbiota dysbiosis aggravated the severity of EAE. MOG_35–55 peptide was adopted for the induction of EAE in female C57BL/6 mice immediately after the fecal collection. Daily clinical scores (a), mean clinical scores (b), and cumulative scores (c) of each group mice. On the 25st day p.i. to initiate EAE, lumbar spinal cords were isolated from the mice for pathological studies. The lumbar spinal cords were stained with HE and LFB to evaluate inflammatory cell infiltration and demyelination, respectively. Representative sections (× 50, scale bars = 500 μm; × 100, scale bars = 200 μm) and statistical analysis of the histopathological degree of the lumbar spinal cords of mice from three different groups are shown (d, e). N = 6 in each group. Data are displayed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001