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Fig. 6 | Journal of Translational Medicine

Fig. 6

From: CYB561D2 up-regulation activates STAT3 to induce immunosuppression and aggression in gliomas

Fig. 6

CYB561D2 knock-down inhibits aggressive tumor behaviors in in vitro assays. A Representative western blots and its quantification showing the protein levels of CYB561D2, total STAT3and pSTAT3 after CYB561D2 knock-down in U251 and U87 cell lines. MTT assay showing the growth curves of U251 (B) and U87 (C) after CYB561D2 knock-down. D Representative images and its quantification of colony formation of U251 and U87 after CYB561D2 knock-down. E Representative images and its quantification of cell migration of U251 and U87 after CYB561D2 knock-down. F RealTime-Glo™ Annexin V Apoptosis assay showing the effects of CYB561D2 knock-down in U251 and U87 cell lines. G Caspase-Glo® 3/7 assay showing the effects of CYB561D2 knock-down in U251 and U87 cell lines. For all, **P < 0.01; ***P < 0.001

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