Fig. 4

Representative example of “bridge joint” rearrangements generating EML4-ALK fusion transcripts from case 9. A DNA sequencing reads indicating EML4-LCLAT1 and LCLAT1-ALK fusion regions were visualized by the IGV software. B RNA sequencing reads indicating EML4 and ALK fusion regions were visualized by the IGV software. C Possible schematic diagram of EML4-LCLAT1 and LCLAT1-ALK fusions that were detected by DNA-based NGS, but a EML4-ALK fusion transcript was identified by RNA-based NGS. Red dashed lines indicate fusion breakpoints and red arrows indicate direction of transcription. D Negative ALK FISH pattern with combined red and green signals. E Positive ALK expression detected by IHC assay