Fig. 3

Co-localisation and immunofluorescent images of TRPM3 with PIP₂ in NK cells of HC. A Example of immunostaining of PIP₂ (red), nucleus (DAPI, blue), TRPM3 (green)) in an NK cell under control IL-2 conditions. Images taken using Nikon A1R + confocal microscopy. B Bar graphs represent correlation between target antigens using Pearson’s correlation coefficient and the degree of overlap between target antigens using Mander’s overlap coefficient. Co-localisation coefficients K1 (TRPM3) and K2 (PIP₂) were used to determine contribution to co-localisation. Number of cells analysed are included within bar graphs: Control n = 287; PregS n = 292; and PregS + Ono n = 237. Data presented as mean ± SEM and *p < 0.05; **p < 0.01. TRPM3, transient receptor potential Melastatin; PIP₂, phosphatidylinositol 4,5-bisphosphate; PregS, pregnenolone sulfate; Ono, ononetin; IL-2, interleukin 2