Fig. 2

Co-localisation and immunofluorescent images of TRPM3 with actin in NK cells of ME/CFS patients. A Example of immunostaining of actin (phalloidin, red), nucleus (DAPI, blue), TRPM3 (green) in NK cell under control IL-2 condition. Cells were stimulated overnight with IL-2 (20 IU) (control) and treated with PregS (30 µM) and ononetin (3 µM). Images taken using Nikon A1R + confocal microscopy. B Bar graphs represent correlation between target antigens using Pearson’s correlation coefficient and the degree of overlap between target antigens using Mander’s overlap coefficient. Co-localisation coefficients K1 (TRPM3) and K2 (actin) were used to determine contribution to co-localisation. Number of cells analysed are included within bar graphs: Control n = 185; PregS n = 193; and PregS + Ono n = 197. Data presented as mean ± SEM. TRPM3, transient receptor potential Melastatin; PregS, pregnenolone sulfate; Ono, ononetin; IL-2, interleukin 2