Fig. 3

NPs increases DR5 expression by inhibiting BIRC5 level. A RT-qPCR validation of BIRC5 expression in lung adenocarcinoma cells treated with different concentrations of NPs (concentrations indicated as concentrations of siBIRC5). B Western blotting result of DR5 expression in lung adenocarcinoma cells treated with different concentrations of NPs. C DR5 expression detected by immunofluorescence staining after NPs treatment of lung adenocarcinoma cells for 24 h. D Lung adenocarcinoma cells labeled with PE-coupled anti-human DR5 (CD262) and the expression of DR5 in lung adenocarcinoma cells determined by flow cytometry. E Western blotting results of DR5 expression in A549 cells