Fig. 2

NPs decreases BIRC5 expression in tumor cells. A BIRC5 expression in tissues from lung adenocarcinoma patients and non-tumor tissues detected by RT-qPCR. B BIRC5 expression in tissues from lung adenocarcinoma patients and non-tumor tissues detected by Western blotting. C BIRC5 protein expression in different groups analyzed by Western blotting. D Colony formation of A549 and H460 cells. E BIRC5 mRNA expression quantified in A549 cells by RT-qPCR after different treatment. F BIRC5 mRNA expression quantified in H460 cells by RT-qPCR after different treatment. G Immunofluorescence staining after different treatment. BIRC5 positive-cells are counted. H, BIRC5 content quantified in A549 and H460 cells by ELISA after different treatment. * indicates p < 0.05