Fig. 3

Differentially expressed genes (DEGs) of CD4⁺ T cell transfected with tRF-3009 and negative control (NC). A The Heatmap of DEGs of CD4⁺ T cell from three healthy controls transfected with tRF-3009 (n  =  3) and NC (n  =  6). The red indicates upregulated genes, and the green indicates downregulated genes. B The Volcano plot of DEGs of CD4⁺ T cell transfected with tRF-3009 (n  =  3) and NC (n  =  6). The red points indicate upregulated genes (n  =  861), and the blue points indicate downregulated genes (n  =  910). C Gene ontology (GO) analysis (left panel) and Pathway analysis (right panel) of DEGs. D Relation network of KEGG pathways enrichment of DEGs. The red indicates pathways that upregulated DEGs significantly enriched, and the blue indicates pathways that downregulated DEGs significantly enriched. E The DEGs related to type I IFN signaling pathway or respiratory electron transport chain. The purple indicates DEGs related to type I IFN signaling pathway, the red indicates DEGs related to respiratory electron transport chain