Fig. 4
From: HLA-dependent heterogeneity and macrophage immunoproteasome activation during lung COVID-19 disease
Single-cell heterogeneity in PSMB8 expression reveals cell trajectory in alveolar M1 macrophages during mild COVID-19. A t-SNE dimensionality reduction performed on transcriptomes of CD14+/CD68+ double-positive bronchoalveolar cells from COVID-19 patients. B Relative abundance of CD14+/CD68+ bronchoalveolar cells grouped according to level of PSMB8 expression during COVID-19 disease. C t-SNE dimensionality reduction performed on transcriptomes of CD14+/CD68+ bronchoalveolar cells from COVID-19 patients, with level of PSMB8 expression indicated by color; D Pseudotime transformation of cell trajectory based on PSMB8 expression in CD14+/CD68+ bronchoalveolar cells from COVID-19 patients. E Pseudotime trajectory expression heatmap of genes whose expression in CD14+/CD68+ cells from COVID-19 patients was linked to the PSMB8-based branch (identified with BEAM3 function in monocle R-package). F Assessment of gene ontology biological process (GO BP) functional enrichment in genes associated with the PSMB8 expression trajectory in CD14+/CD68+ bronchoalveolar cells from COVID-19 patients. G Expression dotplot of markers following the PSMB8 trajectory in CD14+/CD68+ bronchoalveolar cells from COVID-19 patients