Fig. 5

MiR-520d-5p targeted SIK2 in TNBC. A Target mRNAs for miR-520d-5p based on prediction of DIANA and microT databases. B Expression levels of predicted mRNAs in TNBC tissues and adjacent non-tumor tissues were determined by RT-qPCR. C SIK2 expression in TNBC cells and MCF10A cell line was detected via RT-qPCR. D The binding sequence of miR-520d-5p on SIK2 3′UTR was predicted through starBase. E The binding of miR-520d-5p to SIK2 was confirmed by luciferase reporter assay. F RIP assay showed the enrichments of PITPNA-AS1, miR-520d-5p and SIK2 in the beads-conjugated with anti-IgG or anti-Ago2. G The efficiency of miR-520d-5p inhibition in HCC1937 and MDA-MB-468 cells was examined by RT-qPCR. H SIK2 expression in HCC1937 and MDA-MB-468 cells in each group was detected by RT-qPCR and western blotting analyses. **p < 0.01, ***p < 0.001