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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: MYBL2-induced PITPNA-AS1 upregulates SIK2 to exert oncogenic function in triple-negative breast cancer through miR-520d-5p and DDX54

Fig. 4

PITPNA-AS1 acted as a sponge of miR-520d-5p. A Potential miRNAs for PITPNA-AS1 were obtained from starBase. B The binding capacity between PITPNA-AS1 and predicted miRNAs was assessed by RNA pull-down assay. C MiR-520d-5p expression in TNBC samples and adjacent non-tumor specimens was detected through RT-qPCR analysis. D RT-qPCR was utilized for evaluating miR-520d-5p expression in TNBC cell lines and MCF10A cell line. E MiR-520d-5p expression in HCC1937 and MDA-MB-468 cell transfected with miR-520d-5p mimics by RT-qPCR. F A binding site between PITPNA-AS1 and miR-520d-5p was demonstrated. G Luciferase reporter assay validated the binding of PITPNA-AS1 to miR-520d-5p. ***p < 0.001

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Journal of Translational Medicine

ISSN: 1479-5876

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