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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: MYBL2-induced PITPNA-AS1 upregulates SIK2 to exert oncogenic function in triple-negative breast cancer through miR-520d-5p and DDX54

Fig. 2Fig. 2

The biological function of silenced PITPNA-AS1 in vitro. A RT-qPCR analysis was conducted to verify PITPNA-AS1 knockdown efficiency in HCC1937 and MDA-MB-468 cells. B, C CCK-8 and colony formation assays evaluated the proliferative ability of HCC1937 and MDA-MB-468 cells transfected with sh-PITPNA-AS1#1/2 or sh-NC. D Flow cytometry analysis was conducted to detect the effect of PITPNA-AS1 knockdown on HCC1937 and MDA-MB-468 cell apoptosis. E, F Cell migration and invasion in PITPNA-AS1-silenced cells was analyzed by wound healing and Transwell assays. G Levels of proteins related to the EMT process were tested by western blot analysis in sh-PITPNA-AS1#1/2 or sh-NC group. ***p < 0.001

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