ALKBH3 partner ASCC3 mediates P-body formation and selective clearance of MMS-induced 1-methyladenosine and 3-methylcytosine from mRNA

Table 1 Proteins displaying most affected binding to mRNA subsequent to MMS treatment

Gene	Protein name	Anova	t-test significance			Median log₂ SILAC ratios
Gene	Protein name	Anova	0 h	4 h	15 h	0 h	4 h	15 h
Significant differential binding
ZC3HAV1	Zinc-finger CCCH-type antiviral protein 1	+	+			− 0.51	− 0.52	− 0.19
SND1	Staphylococcal nuclease domain-containing protein 1	+	+			− 1.03	− 0.04	− 0.22
HDLBP	Vigilin	+				− 0.65	− 0.11	− 0.16
ZC3H8	Zinc finger CCCH domain-containing protein 8	+				− 0.77	− 0.23	0.27
SERBP1	Plasminogen activator inhibitor 1 RNA-binding protein	+	+			− 1.10	− 0.05	− 0.06
NSUN2	tRNA (Cytosine(34)-C(5))-methyltransferase	+			+	− 0.19	0.15	0.59
RPSA	40S ribosomal protein SA	+	+			− 0.58	− 0.05	0.05
RPS3	40S ribosomal protein 3	+	+			− 0.93	− 0.08	0.00
RPS10	40S ribosomal protein 10	+				− 0.74	0.03	0.07
RPS14	40S ribosomal protein 14	+	+			− 0.91	− 0.07	− 0.05
RPS26P11	Putative 40S ribosomal protein S26-like 1		+			− 0.81	− 0.08	0.05
Sub-significantly increased binding
ASCC3	Activating signal cointegrator 1 complex subunit 3					0.72	− 0.02	0.21
YTHDC2	Probable ATP-dependent RNA helicase YTHDC2					0.56	0.03	− 0.01
TRIM25	E3 ubiquitin/ISG15 ligase TRIM25					0.80	0.05	0.06
GEMIN5	Gem-associated protein 5					0.62	0.78	0.50

ISSN: 1479-5876