Fig. 5
Knockdown of ASCC3 mediates delayed removal of aberrant m1A and m3C, but not m7G, from the mRNA pool. PC-3 prostate cancer cells were subjected to stable lentiviral knockdown of ASCC3. Knockdown and wild type (WT) cells were treated with 1 mM MMS for 1 h (light grey field) and mRNA extracted at various time points. Each data point represents the mean of three biological replicates with SDs as indicated. Removal of MMS-induced (CH3) m1A (A) and m3C (B) was significantly reduced in the ASCC3-deficient cells compared to WT at 4 h and 12 h (m1A) and 4 h (m3C), respectively (* t-test, p < 0.001), whereas no such effect was observed for removal of m7G (C)