Fig. 8

Effect of STAT3 phosphorylation blockade on the development of peritoneal fibrosis. Peritoneal membrane of mice was collected at 28 days after 4.25% HG-PDF injection with or without administration of S3I-201 (10 mg/kg/day). A Photomicrographs illustrated Masson trichrome stain of peritoneal membrane. Original magnification, ×200. B The graph showed the thickness of the compact zone measured from ten random field (original magnification, ×200) of three mice peritoneal samples. C Photomicrographs illustrated immunohistochemical staining of Collagen I in the submesothelial compact zone, and D quantitative data of relative Collagen I positive area (n = 3 per group). Original magnification, ×200. E, F Peritoneal membrane lysates were subjected to immunoblot analysis with specific antibodies against Collagen I, α-SMA, or GAPDH; the representative results with three samples were shown. Data were expressed as the mean ± SE of three experiments. P values were determined by t tests. *P < 0.05; **P < 0.01; ***P < 0.001. G Schematic of the role of STAT3/HIF-1α in peritoneal fibrosis as well as the role of STAT3 inhibitor S3I-201. Scale bar, 50 μm