Fig. 6

The activation of STAT3 pathway in mouse PF model. Peritoneal membrane of mice was collected at 28 days after 4.25% HG-PDF injection with or without administration of S31-201 (10 mg/kg/day). A Photomicrographs illustrated containing of p-STAT3 (green) and α-SMA (red) in the peritoneal membrane. Co-location of p-STAT3 (green) and α-SMA (red) in the peritoneal membrane was showed as white arrows. DAPI, 4^′6-diamidino-2-phenylindole. Original magnification, ×200. B, C Peritoneum lysates were subjected to immunoblot analysis with specific antibodies against p-STAT3, STAT3, or GAPDH; the representative results with three samples were shown. and the relative ratios of p-STAT3 to total STAT3 were expressed as the mean ± SE of three experiments. P values were determined by t tests. *P < 0.05. Scale bar, 50 μm