Fig. 1

c-Fos expression is elevated in IgE-stimulated MCs. A IgE-sensitized RBL-2H3 were stimulated by DNP-HSA for 30 min, β-hexosaminidase released into the supernatant was measured. B–D IgE-sensitized RBL-2H3 were stimulated by DNP-HSA for 4 h, the gene expression of pro-inflammatory cytokines TNF α, Ccl2 and IL 4 was analyzed by RT-PCR. E Heatmap of 36 selected DEGs. F–I The gene expression of Fos family (c Fos, Fos B, Fosl1, Fosl2) was determined by RT-PCR. J Western blot for c-Fos family protein members (c Fos, p-c Fos, FRA 1, Fos B). K Band signals were analyzed by Image J software. Data summarize three independent experiments; *p < 0.05 **p < 0.01 vs. nontreated activated cell