Droplet digital PCR allows vector copy number assessment and monitoring of experimental CAR T cells in murine xenograft models or approved CD19 CAR T cell-treated patients

Table 1 Primer and probe sequences

Primers/probes		Name	Orientation	5′–3′ Sequence	Target area	PCR/Size of product (bp)	Targeted CAR/Genomic DNA
CAR	Primers	CD28j	Fw	CACGTCTCTTGTCCAAAACATC	Junction CD28/CD3z	28z/137	axi-cel; experimental CS1
		41BB	Fw	GAAGAAGAAGAAGGAGGATGTG	41BB	41BBz/145	tisa-cel; experimental IL-1RAP
		CD3z	R	CTTCGCAGCCTATCGCTCCAG	CD3z		Common to all CARs
	Probe [Fam/BHQ]	pCAR	Fw	CCCGCGTACCAGCAGGGCCAGA	CD3z		Common to all CARs
Reference	Primers	fGAPDH	Fw	ACATCATCCCTGCCTCTAC	GAPDH	GAPDH/179	Genomic DNA GAPDH specific
	Primers	rGAPDH	R	CTGCTTCACCACCTTCTTG	GAPDH	GAPDH/179
	Probe [Hex/BHQ]	pGAPDH	Fw	CACTGCCAACGTGTCAGTGGTGGACCT	GAPDH

ISSN: 1479-5876