Fig. 2

A Real time qPCR of DNA plasmids. 28z and 28BBz plasmids were serially diluted in untransduced CEM DNA and amplified by PCR with both Fw28z/Rv3z and GAPDH primer pairs. Amplification curves are provided (left). Ct is plotted against log transgene dilution, and linear regression equations and correlation coefficients are provided (right) for n = 4 independent experiments. B Flow cytometry-based CAR T cell detection. CS1- or IL-1RAP-transduced CEM cell lines were serially diluted in an untransduced CEM cell line. The percentage of the starting transduced cell population is provided. Representative experiment. SSC-A: side scatter. C Real-time qPCR of genomic DNA from serially diluted CAR-transduced cells. Genomic DNA was extracted from CS1- or IL-1RAP-transduced CEM cells serially diluted in untransduced CEM and amplified by 28z, 28BBz and GAPDH qPCR. Amplification curves are provided (left). Ct is plotted against log transgene dilution, and linear regression equations and correlation coefficients are provided (right) for n = 4 independent experiments. RFU: relative fluorescence units; Ct: cycle threshold; Ct: cycle threshold