Fig. 1

A Schematic representation of the CAR constructs and localization of PCR primers and probes. Localization of primers and probe for 28BBz and 28z PCRs specific for experimental IL-1RAP and tisa-cel CARs and for experimental CS1 and tisa-cel CARs, respectively. The sense primer for 28z PCR spans the CD28/CD3z junction area. FMC63: Single chain of the CD19 antibody. scFv: Single chain of the variable fragment. B Agarose gel electrophoresis of the 28z and 28BBz PCR products. MW: molecular weight marker; bp: base pair