Fig. 4

KDM5A inhibits PJA2 expression by removing H3K4me3 and promoted macrophage M2 polarization. A The mRNA expression of PJA2 was detected by RT-qPCR (n = 30). B The mRNA expression of PJA2 in GC-TAMs and Macs of adjacent tissues was detected by RT-qPCR. C KDM5A protein expression was detected by Western blot after KDM5A was silenced in TAMs. D After KDM5A was silenced in TAMs, Western blot was used to detect the protein expression of PJA2. E After KDM5A was silenced in TAMs, the enrichment of H3K4me3 on PJA2 promoter was detected by ChIP assay. F The mRNA and protein expression of M1 and M2 macrophage markers in TAMs was detected by RT-qPCR and ELISA, respectively. *p < 0.05