Fig. 3

ELK4 promotes macrophage M2 polarization through transcriptional activation of KDM5A. A Prediction of ELK downstream target gene. The three circles in the Figure represent the prediction results of ChipBase database, LinkedOmics database and significantly different genes in TCGA and GTEX, and the middle part represents the intersection of the three groups of data. B The interaction analysis of ELK4 downstream target genes. Each small circle in the figure represents a gene, and the line between circles indicates the interaction between genes. A darker circle color indicates a higher number of interacting genes, a higher degree value and a higher core degree in the network graph. C The statistics of the core gene degree value in the gene interaction network graph. The abscissa represents the degree value, and the ordinate represents the gene name. D The correlation analysis results of ELK4 and KDM5A in TCGAGC data. The abscissa represents ELK4 expression, and ordinate represents KDM5A. Each small circle in the graph represents a sample, and the person correlation coefficient and correlation p value are shown above. E The expression of KDM5A in GC data collected by TCGA and GTEX (T: Tumor. N, Normal. *p < 0.01). F The mRNA expression level of KDM5A in GC tissues and adjacent tissues of GC patients (n = 30). G RT-qPCR was used to detect KDM5A mRNA expression in GC-TAMs and in Macs of adjacent tissues. H After silencing ELK4 in TAMs, the protein expression of ELK4 was detected by Western blot. I After ELK4 was silenced in TAMs, the protein expression of KDM5A was detected by Western blot. J After ELK4 was silenced in TAMs, the effect of ELK4 on KDM5A activity was detected by dual luciferase reporter gene assay. K, ELK4 was silenced in TAMs, ChIP assay was used to detect the enrichment of ELK4 on KDM5A promoter. L RT-qPCR and ELISA were used to detect the mRNA and protein expression of M1 and M2 macrophage markers in TAMs. *p < 0.05