Fig. 6

MEG3 suppresses immune escape by decreasing regulatory T cell differentiation through p53/miR-149-3p/FOXP3 axis. Normal mice were used as controls, and EC mice were treated or not treated with oe-NC, oe-MEG3, oe-MEG3 + sh-NC, oe-MEG3 + sh-p53, oe-MEG3 + oe-NC or oe-MEG3 + oe-FOXP3. A The weight of mice. B Pathological changes of tissues in mice measured by HE staining (200×). C Immunohistochemistry result of detecting FOXP3, C-myc, N-myc and Ki-67 protein expression. D MEG3, p53, miR-149-3p and FOXP3 expression in mice tissues detected by RT-qPCR normalized to GAPDH and U6. E The percentage of CD25⁺FOXP3⁺ T cells in CD4⁺ T cells in mice spleen assessed by flow cytometry. F The percentage of IL-10⁺CD4⁺ T cells in mice spleen assessed by flow cytometry. G The percentage of IL-4⁺CD4⁺ T cells in mice spleen assessed by flow cytometry. H IL-10 level in the spleen of mice assessed by ELISA. I IL-4 level in the spleen of mice assessed by ELISA. * p < 0.05 vs. EC mice treated with oe-NC. # p < 0.05 vs. EC mice treated with oe-MEG3 + sh-NC. $ vs. EC mice treated with oe-MEG3 + oe-NC. Measurement data were expressed as mean ± standard deviation. Data between two groups were compared by independent sample t test. Comparisons among multiple groups were performed using one way analysis of variance (ANOVA), followed by Tukey's post hoc test, and data at different time points among multiple groups were compared by repeated measures ANOVA, followed by Bonferroni post hoc test. n = 10