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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Long non-coding RNA MEG3 mediates the miR-149-3p/FOXP3 axis by reducing p53 ubiquitination to exert a suppressive effect on regulatory T cell differentiation and immune escape in esophageal cancer

Fig. 4

p53-activated miR-149-3p results in suppression of regulatory T cell differentiation and immune escape in EC mice via FOXP3. A p53 expression in cancer and paracancerous tissues detected by RT-qPCR normalized to GAPDH (n = 56). * p < 0.05 vs. paracancerous tissues. B p53 expression in AKR cells detected by RT-qPCR normalized to GAPDH. * p < 0.05 vs. control cells. C p53 and miR-149-3p expression in AKR cells after overexpression of p53 detected by RT-qPCR normalized to GAPDH and U6. * p < 0.05 vs. AKR cells treated with oe-NC. D The enrichment of p53 in miR-149-3p promoter measured by ChIP. * p < 0.05 vs. IgG. Normal mice were used as controls, and EC mice were treated or not treated with oe-NC, oe-p53, oe-p53 + NC-inhibitor or oe-p53 + miR-149-3p-inhibitor. E The weight of mice (n = 10). F FOXP3 expression in mice tissues detected by RT-qPCR normalized to GAPDH (n = 10). G Pathological changes of mice tissues measured by HE staining (200 ×) (n = 10). H Immunohistochemistry result of detecting FOXP3, C-myc, N-myc and Ki-67 protein expression (n = 10). I The percentage of CD25+FOXP3+ T cells in CD4+ T cells in mice spleen assessed by flow cytometry (n = 10). J The percentage of IL-10+CD4+ T cells in mice spleen assessed by flow cytometry (n = 10). K IL-10 level in mice spleen assessed by ELISA (n = 10). L The percentage of IL-4 + CD4 + T cells in mice spleen assessed by flow cytometry (n = 10). M IL-4 level in mice spleen assessed by ELISA (n = 10). * p < 0.05 vs. EC mice treated with oe-NC. # p < 0.05 vs. EC mice treated with oe-p53 + NC-inhibitor. Measurement data were expressed as mean ± standard deviation. Data between cancer and paracancerous tissues were compared by paired t test, and data between other two groups were compared by independent sample t test. Comparisons among multiple groups were performed using one way analysis of variance (ANOVA), followed by Tukey's post hoc test, and data at different time points among multiple groups were compared by repeated measures ANOVA, followed by Bonferroni post hoc test. The cell experiment was repeated 3 times

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