Fig. 1

Silencing of FOXP3 inhibits regulatory T cell differentiation to repress immune escape in EC mice. A FOXP3 expression in cancer and paracancerous tissues detected by RT-qPCR normalized to GAPDH (n = 56). * p < 0.05 vs. paracancerous tissues. B The percentage of CD25⁺FOXP3⁺ T cells in CD4⁺ T cells of peripheral blood from EC patients and healthy blood donors assessed by flow cytometry (EC patients: n = 28; healthy blood donors: n = 25). * p < 0.05 vs. peripheral blood from healthy blood donors. C FOXP3 expression in AKR cells detected by RT-qPCR normalized to GAPDH. * p < 0.05 vs. control cells. D The silencing efficiency of sh-FOXP3-1 and sh-FOXP3-2 in AKR cells detected by RT-qPCR normalized to GAPDH. * p < 0.05 vs. AKR cells transfected with sh-NC. Normal mice were used as controls, and 4-NQO-induced mice were transfected or not transfected with sh-NC or sh-FOXP3. E The weight of mice (n = 10). F Pathological changes of tissues in mice measured by HE staining (200×) (n = 10). G FOXP3 expression in tissues of mice detected by RT-qPCR normalized to GAPDH (n = 10). H FOXP3, C-myc, N-myc and Ki-67 positive protein expression in tissues of mice determined by immunohistochemistry (n = 10). I The percentage of CD25⁺FOXP3⁺ T cells in CD4⁺ T cells in spleen of mice assessed by flow cytometry (n = 10). J The percentage of IL-10⁺CD4⁺ T cells in spleen of mice assessed by flow cytometry (n = 10). K IL-10 level in the spleen of mice assessed by ELISA (n = 10). L The percentage of IL-4⁺CD4⁺ T cells in spleen of mice assessed by flow cytometry (n = 10). M IL-4 level in the spleen of mice assessed by ELISA (n = 10). * p < 0.05 vs. normal mice. # p < 0.05 vs. EC mice treated with sh-NC. Measurement data were expressed as mean ± standard deviation. Data between cancer and paracancerous tissues were compared by paired t test, and data between other two groups were compared by independent sample t test. Comparisons among multiple groups were performed using one way analysis of variance (ANOVA), followed by Tukey's post hoc test, and data at different time points among multiple groups were compared by repeated measures ANOVA, followed by Bonferroni post hoc test. The cell experiment was repeated 3 times