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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: Oxidative stress response in regulatory and conventional T cells: a comparison between patients with chronic coronary syndrome and healthy subjects

Fig. 1

The response to oxidative stress ex vivo in Treg and Tconv cells. A Percentages of living Treg and Tconv cells, following treatment with 5, 10, 20, or 30 µM H2O2 for 18 h. % of living cells is defined as negative for Annexin-V and SYTOX and normalized based on untreated cells (medium only). Asterisks indicate significant differences between Treg and Tconv cells, all p < 0.0001. Number of study subjects per dose of H2O2 were n = 19 for 5 µM, n = 33 for 10 µM, n = 22 for 20 µM, n = 34 for 30 µM. B The levels of oxidative stress, measured as mean fluorescence intensity (MFI) of CellROX Green, is shown in Treg and Tconv cells following treatment with 0, 10, 30, 60 or 120 µM H2O2 for 2 h (B). Asterisks indicate significant differences between Treg and Tconv cells, all p < 0.0001 (except for 60 µM H2O2, p < 0.001). Number of study subjects per dose of H2O2 were n = 22 for 0 µM, n = 3 for 10 µM, n = 20 for 30 µM, n = 20 for 60 µM, n = 12 for 120 µM. Box plots display median and i-q range values

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