Fig. 5

PNA-A15 suppresses CSC-like behaviour of human lung cancer in H460, H292, H23 and A549 cells. a H460, b H292, c H23, and d A549 cells were treated with PNA-A15 or scramble (SC) 5 μM) for 48 h. After treatment, cells were grown in a 24-well ultralow attachment plate at a density of 2.5 × 10³ cells/well for 7 days to form primary spheroids. These primary spheroids were trypsinized as single cells and replated for 14 days in a 24-well ultralow attachment plate to form secondary spheroids. At day 14, the number and size of the secondary spheroids were determined and imaged using phase-contrast microscopy (Olympus IX51 with DP70). The relative spheroid number and size of PNA-A15- or scramble-treated cells were compared to those of untreated controls. All plots present the mean ± SD (n = 3). *P < 0.05 vs. untreated cells