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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: Targeting multiple genes containing long mononucleotide A-T repeats in lung cancer stem cells

Fig. 1

The expression of down- and upregulated genes containing A and T repeats (X-axis) with the repeat density in bp/Mbp (Y-axis). a Lysates of parental cells and CSC-enriched cells were prepared and analysed for CD133, CD44, ABCG2 and ALDH1A1 expression by Western blotting. The GSE142616 microarray dataset was downloaded from GEO. Only perfect A and T repeats were classified as downregulated (Dn), neutral (Nu), or upregulated (Up) genes. Experimental and control samples were the spheroid and monolayer of b H292 and c A549 cell lines, respectively. d Venn diagram shows overlap between the intersection of downregulated genes between H292 (the left circle) and A549 (the right circle) cell lines. e The list of candidate genes determined by biological process from Gene Ontology. Student's t-test P-values, which are denoted by P, indicate that the mean difference between Dn/Up and Nu is statistically significant. All plots present the mean ± SD (n = 3). *P < 0.05 vs. untreated cells

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