Fig. 4

Prediction and initial validation of the target genes of miR-20a. A The potential target genes of miR-20a predicted by TargetScan, microT-CDS, and miRDB. 532 overlapping targets predicted by the three online programs were then functional enrichment analyzed. B a GO slim summary for the 532 target genes annotated by the online WebGestalt tool. C Functional annotation of the 532 overlapping genes based on KEGG pathway Database by the online WebGestalt tool. D The mRNA expression levels of 7 selected potential target genes in EAE and control mice (n = 6–9). Data are shown as mean ± SEM. * p < 0.05, *** p < 0.001 using unpaired Student’s t-test