Fig. 1

Presence of IL-24 and IL-20RB in IBD. Protein level of IL-24 in the colonic mucosa (n = 7–10) of children with CD, UC and controls was determined by Western blot analysis in comparison with GAPDH as internal control (a, c). Concentration of IL-24 in the serum (n = 12–15) of controls and children with CD was measured by ELISA (b). Violin plots indicate the distribution of IL-24 levels in the given groups. Relative mRNA expression of IL24 in PBMCs (d) and LPMCs (e) after treatment with IL-1ß, LPS, TNF-α, TGF-ß1, IL-17 and H₂O₂ (n = 6) was measured by real-time PCR in comparison with GAPDH as internal control. Results are presented as mean ± SD. *p < 0.05 vs. control (Kruskal–Wallis test (a, d, e) or Mann–Whitney U-test (b)). Localization of IL-20RB (red) and αSMA (green) was determined by immunofluorescence staining of the colonic mucosa of controls and children with IBD (f). Nuclei are stained with Hoechst33342 (blue). Scale bars: 100 µm and 50 µm