Table 1 Primer sequences for qRT-PCR. The primers used for MALAT1, NEAT1, and H19 were previously published, while the primers for TP73-AS1, ABALON, and HOXA10-AS were designed using primers3 (http://www.ncbi.nlm.nih.gov/tools/primer-blast/)
Primer | Sequence |
|---|---|
MALAT1 [25] | F:GAATTGCGTCATTTAAAGCCTAGTT |
R:GTTTCATCCTACCACTCCCAATTAAT | |
NEAT1[26] | F: GTGGCTGTTGGAGTCGGTAT |
R: ACCACGGTCCATGAAGCATT | |
TP73-AS1 | F:CCGGTTTTCCAGTTCTTGCAC |
R:GCCTCACAGGGAAACTTCATGC | |
ABALON | F:CCCCCTCCAGGTACCAGAAC |
R:CCACTGGTGCTTTCGATTTGA | |
H19 [27] | F:TCAGCTCTGGGATGATGTGGT |
R:CTCAGGAATCGGCTCTGGAAG | |
HOXA10-AS | F: CCCAGTAAGCCAAAGTCAAGCC |
R: CTGAGGTCAATGGTGCAAAGG |