Fig. 3
Overexpression of METTL13 inhibits proliferation, viability, migration and invasion of ccRCC cells in vitro. a METTL13 protein levels in ccRCC cells (OS-RC-2 and ACHN cells) respectively transfected with METTL13 expressing plasmid (METTL13) and control vector (vector) confirmed by western blotting. b Cell proliferation was detected by EdU assay. c CCK-8 assay was performed to measure cell viability. d Cell migration rate was observed via wound-healing assay. e Migratory ability and invasiveness of ccRCC cells were detected by migration assay and invasion assay, respectively. f EMT-related protein levels were detected by performing western blotting. Each experiment was conducted independently triplicate. Student’s t-test (two-tailed) was used for statistical analysis, *P < 0.05, **P < 0.01, ***P < 0.001. Bar graphs: mean ± S.D., n = 3