Fig. 4

Knockdown of eIF6 inhibited the proliferation and invasion of HCC cells in vitro. a Western blot analysis identified the efficiency of knockdown eIF6 lentiviruses in HCC cells. b The blot density of the western blotting was quantified using the ImageJ software. β-actin was used as the loading control. c, d CCK-8 assays were used to determine the cell proliferation capacity in HepG2 and Huh-7 cells infected with knockdown eIF6 lentiviruses. e Plate colony formation assays of NC cells and Sh-eIF6 cells (left). The number of colony formation was counted after 15 days (right). f Transwell-Matrigel invasion assays of NC cells and Sh-eIF6 cells (left). The number of cells invaded after 24 h was counted in five randomly selected microscopic fields (right). Bar = 100 μm. The error bar represents the mean ± SD (n = 3). *p < 0.05; **p < 0.01; and ***p < 0.001