Fig. 3
Expression of PRICKLE1 in primary AML samples and cell lines. a qRT-PCR analysis of PRICKLE1 mRNA expression in AML patient samples (n = 129) and normal controls (n = 12). b PRICKLE1 mRNA expression in patients with newly-diagnosed AML (n = 129), patients with relapsed AML (n = 13) and patients with complete remission (n = 35). c qRT-PCR analysis of 5 patients with AML at the time of newly diagnosed, complete remission and relapse. d Representative western blotting analysis of PRICKLE1 in patients with AML (n = 6) including patients with extramedullary metastasis (n = 3) relative to control (n = 2). e, f qRT-PCR and western blotting analysis of PRICKLE1 expression in AML cell lines. GM12878 cell line was used as the control group. *P < 0.05; **P < 0.01; ***P < 0.001; NS: not significant