Fig. 7

UCA1 acts as a ceRNA of miR-122-5p to affect SOX2 expression. a Prediction of subcellular localization of UCA1 by online analysis website. b FISH assay verified the subcellular localization of UCA1. c Prediction of binding sites between UCA1 and miR-122-5p in RNA22 website. d Dual luciferase reporter gene assay detected the binding site of UCA1 to miR-122-5p. e RNA pull-down assay verified the enrichment of UCA1 to miR-122-5p. f Prediction of the target relationship between miR-122-5p and SOX2 by online website. g Dual luciferase reporter gene assay detected the target relationship between miR-122-5p and SOX2. * P < 0.05 vs. the mimic-NC group, # P < 0.05 vs. the Bio-miR-122-5p-Wt group. Measurement data were depicted as mean ± standard deviation, comparisons between two groups were assessed by independent sample t test, and comparisons among multiple groups were assessed by one-way analysis of variance followed with LSD-t, the experiment was repeated three times