Fig. 6
From: Reovirus enhances cytotoxicity of natural killer cells against colorectal cancer via TLR3 pathway
TBK1/IKKε mediates NK cell response to reovirus. a NK cells were activated with 10 MOI reovirus or 1 μg/mL Poly (I:C) in the presence or absence of TBK1/IKKε inhibitor BX795 (10 μM) for 12 h, then co-cultured with DLD-1 cells at E:T ratio of 5:1 for 4 h. Cytotoxicity was measured by CCK-8 assays. Data represent means ± SD. b-c Representative histograms of CD69 expression and the fold increase in MFI for CD69 in NK cells determined by flow cytometry d Perforin and IFN-γ in the supernatants were quantified by ELISA. *p < 0.05, **p < 0.01.and the fold increase in MFI for CD69