Fig. 5

TLR3 is required for NK cell response to reovirus. NK cells were stimulated with 1, 10, or 100 MOI reovirus for 12 h. Untreated NK cells were used as a control. a Fold changes in TLR3 gene expression of NK cells relative to control cells, measured by qPCR. b Western blot analysis of TLR3 expression of NK cells. c NK cells were transfected with siTLR3, and knockdown NK cells were then stimulated with 10 MOI reovirus or 1 μg/mL Poly(I:C) for 12 h. Cytotoxicity of control and TLR3 knockdown NK cells against DLD-1 cells was measured by CCK-8 assay. d NK cells were activated with 10 MOI reovirus or 1 μg/mL Poly(I:C) in the presence or absence of 10 μM TLR3/dsRNA complex inhibitor for 12 h. Cytotoxicity of chemically inhibited and control NK cells against DLD-1 cells was measured by CCK-8 assay. e TNF-α and IFN-γ levels in culture supernatants were quantified by ELISA. Error bars represent means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001