Fig. 5

a THP-1 and HL-60 cells were treated with ST1326 and ABT199, alone or combined, for 24 h. qPCR analysis of Mcl-1 expression. b THP-1 and HL-60 cells were pretreated with 1 μM MG132 (proteasome inhibitor) for 6 h, and then incubated with ST1326 for 18 h. Western blot analysis was conducted for Mcl-1 protein levels. c THP-1 and HL-60 cells were treated with ST1326 and ABT199, alone or combined, for 24 h. Western blot analysis was conducted for p-GSK3β, GSK3β, AKT, p-AKT protein levels (d). THP-1 and HL-60 cells were treated with ST1326 and ABT199, alone or combined, for 24 h. Western blot analysis was conducted for p-ERK, ERK, p-Mcl1 Thr163 protein levels. e THP-1 and HL-60 cells were treated with increasing doses of PD98059 for 24 h. Western blot analysis was conducted for p-ERK, ERK, p-Mcl1 Thr163, Mcl-1 protein levels