Fig. 6

LDHA inhibition blocked cellular energy metabolism in NSCLC. a, b A549 and H1975 cells were incubated with oxamate (20 mM) for 24 h followed by 6 Gy irradiation, after 24 h, the lactic acid levels in culture medium was tested in each group. c, d Under the above experiment conditions, intracellular pyruvate levels were tested in A549 and H1975 cells. e Intracellular ATP contents were detected in 549 cells. Cells were incubated with oxamate (20 mM) for 24 h followed by 6 Gy irradiation, after 24 h, ATP levels were assayed, and results were expressed as a percentage of the control group. f N-acetylcysteine (NAC) partly reversed the inhibitive effect of oxamate combined with irradiation. *p < 0.05, compared to the control group; ^#p < 0.05, compared to the irradiation group