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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: Antitumor effect of IL-12 gene-modified bone marrow mesenchymal stem cells combined with Fuzheng Yiliu decoction in an in vivo glioma nude mouse model

Fig. 3

Fluorescent distribution characteristics of IL-12-transfected BMSCs and induction of apoptosis measured by DAPI staining in mice (×400). (Aa), (Ba) and (Ca) indicate DAPI staining results in the BMSC, IL-12 + BMSC and FYD + IL-12 + BMSC groups. (Ab), (Bb) and (Cb) indicate the fluorescence data in the BMSC, IL-12 + BMSC and FYD + IL-12 + BMSC groups. (Ac), (Bc) and (Cc) indicate DAPI staining and fluorescence merged signals in the same field using confocal microscopy for the BMSC, IL-12 + BMSC and FYD + IL-12 + BMSC groups. Fluorescence was not observed in the tumor tissue of mice in the BMSC group, whereas it was evident in the tumor tissues from the IL-12 + BMSC and FYD + IL-12 + BMSC groups, indicating that the IL-12 gene was successfully transfected into BMSCs (ac). The induction of apoptosis was measured by DAPI staining. Apoptosis was more prevalent in the IL-12 + BMSC and FYD + IL-12 + BMSC groups compared with that of the BMSC group. The FYD + IL-12 + BMSC group exhibited a higher number of apoptotic cells showing blue fluorescence staining of cells compared with that of the IL-12 + BMSC group. The IL-12 + BMSC group exhibited a higher proportion of apoptotic cells as determined by blue fluorescence staining compared with the BMSC group (ac). The results indicated that the IL-12 gene was distributed in tumor tissues, suggesting a homing effect of BMSCs with regard to IL-12. BMSCs, bone marrow mesenchymal stem cells; DAPI, 4′,6-diamidino-2-phenylindole; FYD, FuzhengYiliu decoction IL-12 + BMSC, IL12-lentiviral-transfected BMSC; BMSC group, empty lentiviral vector-transfected BMSC group; FYD + IL-12 + BMSC group, FYD treatment in IL-12 lentiviral-transfected BMSC group

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