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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Src is essential for the endosomal delivery of the FGFR4 signaling complex in hepatocellular carcinoma

Fig. 4

Co-localization of signaling molecules in the nucleus. a Western blot analysis of the anti-Src antibody co-immunoprecipitate in the nuclear fractions of the three HCC cell lines. Src co-immunoprecipitated with STAT3, but not with FGFR4. b Immunofluorescence staining of FGFR4, pSrc, and pSTAT3 in SNU878 cells. FGFR4 and pSTAT3 were co-localized in the cytosol, but not in the nucleus. pSrc and pSTAT3 were co-localized in the cytosol and nucleus. c Schematic model depicting the endosomal delivery of the FGFR4 signaling complex. FGF19 activates FGFR4, which forms an endosomal complex with pSTAT3 and pSrc, and is transported to the nucleus. d Effects of dasatinib on Src phosphorylation. The level of Src phosphorylation decreased with dasatinib treatment. eā€“h Viability of HCC cells treated with the Src inhibitor, dasatinib, or with the FGFR4 inhibitor BLU9931 (**Pā€‰<ā€‰0.0001). The cytotoxic efficacy of dasatinib was comparable to that of BLU9931

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