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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: Src is essential for the endosomal delivery of the FGFR4 signaling complex in hepatocellular carcinoma

Fig. 3

FGFR4 forms an endosomal complex with Src and STAT3, which transfers signals to the nucleus. a Western blot analysis of the FGFR4 co-immunoprecipitate. FGFR4 was co-immunoprecipitated with EEA1, Src, and STAT3 in the three HCC cell lines. b Western blot analysis of the FGFR4 co-immunoprecipitate following Src silencing. Src silencing substantially decreased the amounts of EEA1 and STAT3 that co-immunoprecipitated with FGFR4. c Immunofluorescence staining of FGFR4 and EEA1 in HUH7 cells treated with either Src siRNA or control siRNA. FGFR4 and EEA1 did not co-localize following Src silencing. d Immunofluorescence staining of FGFR4 and pSTAT3. FGFR4 and pSTAT3 were co-localized in the cytosol and localized in the nucleus of cells treated with the control siRNAs. The levels of FGFR4 and pSTAT3 were substantially reduced in the cytosol and nucleus of the cells that were treated with Src siRNA. e Immunofluorescence staining of pSrc and pSTAT3. It was observed that pSrc and pSTAT3 were co-localized in the cytosol and nucleus of the cells that were treated with the control siRNAs. The levels of pSrc and pSTAT3 were substantially reduced in the cytosol and nucleus of cells treated with Src siRNA

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