Fig. 5

MALAT1 reduced the miR-202-3p expression to upregulate SRSF1. a The binding site between MALAT1 and miR-202-3p predicted by LncBase. b The interaction between MALAT1 and miR-202-3p verified by RNA pull-down experiment. c RIP detection of the binding of MALAT1 to miR-202-3p. d Venn diagram displaying the intersection of the downstream genes of miR-202-3p predicted by TargetScan, RAID, mirDIP, and miRWalk and the top 500 differentially expressed genes in the STAD dataset of TCGA. e PPI network of the obtained genes through intersection constructed by String, wherein red represents high core degree and blue represents low core degree of the genes, of which the reddest gene is the most core. f TargetScan prediction of the binding site between miR-202-3p and SRSF1. g RNA pull-down experiment verifying the interaction between miR-202-3p and SRSF1. h RIP detection of the binding of miR-202-3p to SRSF1. i Luciferase assay detecting luciferase activity in each group after transfection. j RT-qPCR determination of the miR-202-3p expression in each group. k RT-qPCR determination of the SRSF1 expression in each group. l RT-qPCR determination of the MALAT1, miR-202-3p, and SRSF1 expression in each group. *p < 0.05 compared with treatment of Bio NC probe, IgG, mimic NC, and oe-NC + si-NC. #p < 0.05 compared with treatment of oe-MALAT1 + si-NC. The values in the figure were all measurement data, expressed as the mean ± standard deviation. Data between the two groups were compared by unpaired t-test and data between multiple groups by one-way ANOVA and Tukey's post hoc test. The experiment was repeated for three times independently