Fig. 3

CCL21 increased the expression of MALAT1, thus promoting EMT, migration, and invasiveness of SGC-7901 cells. a RT-qPCR determination of the relative expression of MALAT1 in GC tissues and adjacent normal tissues (N = 115). b RT-qPCR measurement of the MALAT1 expression in SGC-7901 cells treated with different concentrations of CCL21 for 48 h and with 150 μg·L⁻¹ CCL21 for different times. c Assessment of the efficiency of transfecting SGC-7901 cells with MALAT1. d Western blot analysis of the effect of CCL21 on the EMT-related factors of SGC-7901 cells after oe-MALAT1 treatment normalized to GAPDH. e The migration and invasion capacities of SGC-7901 cells after oe-MALAT1 treatment assessed by Transwell assay. f RT-qPCR determination of the expression of MALAT1 in SGC-7901 cells after si-MALAT1-1 or si-MALAT1-2 treatment. g Assessment of the efficiency of transfecting SGC-7901 cells with CCL21 after oe-CCL21, si-MALAT1-1, or si-MALAT1-2 treatment. h Western blot analysis of the expression of E-cadherin, Vimentin, Slug, Snail, and Twist after oe-CCL21, si-MALAT1-1, or si-MALAT1-2 treatment normalized to GAPDH. i The number of migratory cells and invaded cells in each group determined by Transwell assay after oe-CCL21, si-MALAT1-1, or si-MALAT1-2 treatment. * p < 0.05 compared with treatment of 0 μg·L⁻¹ CCL21, oe-NC, or si-NC. # p < 0.05 compared with treatment of si-NC + oe-CCL21. Data in the figure were all measurement data, expressed as the mean ± standard deviation. Data between the two groups of GC tissues and adjacent normal tissues were compared by paired t-test, data between the other two groups by unpaired t-test, and data between multiple groups by one-way ANOVA and Tukey's post hoc test. The experiment was repeated for three times independently