Fig. 2

CCL21 promoted migrative and invasive potential as well as EMT of SGC-7901 cells. a RT-qPCR determination of the CCL21 expression in GES-1, SGC-7901, MGC-803, and MKN28 cells. b Results of scratch wound assay. c Representative microscopic images of control SGC-7901 cells and the cells treated with CCL21 (150 μg·L⁻¹) for 48 h (the arrow points to pseudopodium formation) (× 400). d Western blot analysis of the expression of E-cadherin, Vimentin, Slug, Snail, and Twist after CCL21 (150 μg·L⁻¹) treatment normalized to GAPDH. * p < 0.05 compared with GES-1 cells, blank cells or oe-NC-treated cells. The values in the figure were all measurement data, expressed as the mean ± standard deviation. Unpaired t-test was used for comparing data of two groups, and one-way ANOVA was applied for comparing data of multiple groups followed by Tukey's post-test. The experiment was repeated for three times independently