Fig. 4

Effects of antibiotics treatment (ABX) on polarization of macrophages in BPD mice. a The iNOS and Arg-1 expressions were evaluated by western blot and relative gray intensity to β-actin was calculated. b Lung sections were stained by double immunofluorescence with anti-CD68 (macrophage marker, green), anti-Arg-1 (M2 marker, red) and anti-iNOS (M1 marker, red) antibodies. *P < 0.05, **P < 0.01. Results were analyzed using ANOVA