Fig. 6
Effect of circPLXNB2 expression on PLXNB2, apoptosis and proteins associated with cell cycle in vivo. a TUNEL-positive cells were measured in 3 randomly chosen fields from each biopsy (3 × 104 μm/field). qRT-PCR was used to detect the expression of circPLXNB2 (b) and the PLXNB2 mRNA (c) in vivo. d IHC staining of xenograft tumour tissues obtained from three mice randomly selected from each group (n = 6). Representative IHC images with an original magnification of 400× show the expression of PLXNB2 in different murine AML xenograft models. e Image software measured the average optical density of the selected area in sections subjected to IHC staining for PLXNB2. f Representative Western blots showing the expression of BCL2, BAX, cyclin D1 and PLXNB2 in different AML xenograft models. Western blotting was performed on the xenograft tumour tissues obtained from three mice randomly selected from each group (n = 6) and β-actin was used as a loading control. The vector or shRNA NC was used as a control. Each experiment was repeated three times. **P < 0.01 and ***P < 0.001. CircPLXNB2 OE circPLXNB2 overexpression, circPLXNB2 shRNA circPLXNB2 short hairpin RNA