Fig. 3

CircPLXNB2 promotes the proliferation and migration of AML cells and inhibits apoptosis in vitro. Cell proliferation was measured with the CCK-8 method. Apoptosis was analysed with FCM analysis. A Transwell assay was performed to detect cell migration. CircPLXNB2 OE significantly increased the proliferation rates (a) and migration (c) of HL-60 cells, and decreased the number (b) of apoptotic HL-60 cells. The circPLXNB2 shRNA significantly decreased the proliferation rates (d) and migration (f) of OCI-AML3 cells, and increased the number (e) of apoptotic OCI-AML3 cells. g Representative FCM plots of the effects of circPLXNB2 expression on apoptosis. Q2-4: early apoptotic cells; Q2-2: late apoptotic cells. The combined percentages of apoptotic cells in the Q2-4 and Q2-2 quadrants represent the percentage of total apoptotic cells. Nontransfected cells were used as a control, and the vector or shRNA NC was used as a negative control. Each experiment was repeated three times. ***P < 0.001 compared with the control. CircPLXNB2 OE circPLXNB2 overexpression, circPLXNB2 shRNA circPLXNB2 short hairpin RNA, NC negative control, PI propidium iodide